Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina

Bee pollen is the result of agglutination grains collected from flowers and mixed with nectar salivary secretions by honey bees. a natural product exposed to environmental conditions also provides unique microhabitat for yeasts bacterial communities. We analyzed 30 fresh bee samples obtained main producing areas Argentina identify aerobic-spore-forming bacteria. 73 isolates belonging 16 different species through isolation on selective differential media, morphological biochemical tests, PCR RFLP analysis genes encoding 16S rRNA. Our data revealed that Bacillus cereus sensu stricto was most predominant (50%), followed megaterium (40%) subtilis (40%), respectively. In minor proportion, Paenibacillus polymyxa (20%), larvae (17%), pumilus (13%), licheniformis amyloliquefaciens (10%), Lysinibacillus sphaericus (7%), coagulans Rummelliibacillus stabekisii thuringiensis clausii (3%), alvei simplex amylolyticus (3%) were found. results showed Argentinean could transmit diseases due presence viable spores P. toxicogenic B. s.s. strains.